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Evaluation of Apheresis Platelet Concentrates Collected with a Reduced (30‐ml) Collection Chamber with Resuspension and Storage in a Synthetic Medium
Author(s) -
Holme S.,
Heaton W.A.,
Smith K.T.,
Buchholz D.H.
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb01650.x
Subject(s) - platelet , apheresis , plateletpheresis , chemistry , blood product , blood preservation , platelet concentrate , lactate dehydrogenase , zoology , chromatography , medicine , surgery , andrology , biology , biochemistry , enzyme
Recently, the CS‐3000® Plus Blood Cell Separator with the TNX‐6 platelet separation chamber insert has been furnished with a small‐volume (30‐ml) collection chamber. In this study, a platelet synthetic medium containing glucose and bicarbonate (PSM) was used for resuspension and storage of this highly concentrated platelet product. Eighteen donors participated in a paired study design where each participant donated platelets on two occasions, once following collection in a standard chamber with resuspension and storage in plasma and once following collection in the new chamber with resuspension and storage in PSM. Substantially higher total platelet counts were obtained using platelets collected in the small chamber and stored in PSM as compared to control (4.4±0.9times10 11 vs. 3.5±0.9times10 11 platelets, p<0.01 by paired t test). After 5 days of storage, PSM‐stored platelets demonstrated higher ATP levels, less lactate dehydrogenase in the supernatant and increased lactate production with resulting lower pH at day 5 of storage (6.94±0.15 vs. 7.08±0.09, p<0.05). There were no statistically significant differences of the survival by multiple‐hit estimation of PSM‐stored as compared to plasma‐stored platelets as determined by 111 In labeling and infusion. A slight decrease in the initial percent recovery with the additive‐suspended as compared to suspended plasma cells was noted: 50±8 versus 54±9%, respectively (p<0.05). In conclusion, the CS‐3000 Plus/TNX‐6 apheresis system with a new reduced‐volume collection chamber and an additive solution provides a plasma‐poor and highly concentrated platelet product with satisfactory in vivo viability and in vitro functional characteristics after 5 days of storage.