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A New Private Platelet Antigen, Gro a, Localized on Glycoprotein IIIa, Involved in Neonatal Alloimmune Thrombocytopenia
Author(s) -
Simsek Suat,
Vlekke André B.J.,
Kuijpers Robert W.A.M.,
Goldschmeding Roel,
Borne Albert E. G. Kr.
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb01256.x
Subject(s) - antigen , neonatal alloimmune thrombocytopenia , platelet , antibody , immunology , monoclonal antibody , platelet membrane glycoprotein , medicine , immunofluorescence , population , isoantibodies , thrombasthenia , microbiology and biotechnology , biology , genetics , pregnancy , fetus , environmental health , platelet aggregation
The serum of a Caucasian woman who gave birth to a child with neonatal alloimmune thrombocytopenia contained antibodies directed against a platelet antigen of the newborn. There was no incompatibility for the known platelet alloantigens HPA‐1 to HPA‐7 or for the private or low‐frequency antigens Sr a and Va a , between the platelets of the parents. However, crossmatching with the serum of the mother and the platelets of the child and the father was strongly positive, suggesting a new platelet antibody specificity. To investigate the inheritance of the ‘Gro a ’ antigen involved, the available family members were tested in the platelet immunofluorescence test (PIFT) and the monoclonal antibody‐specific immobilization of platelet antigens (MAIPA) assay. The Gro a antigen was found to be inherited in an autosomal‐codominant fashion. In the MAIPA, we localized the Gro a antigen on the glycoprotein IIb/IIIa complex (αIIbβ3). The GP IIb/IIIa localization was confirmed in immunoprecipitation studies. In Western blotting experiments, we further localized the Gro a antigen on the GP IIIa (β3) subunit of the GP IIb/IIIa complex. Until now we have tested approximately 400 unrelated donors. None of these appeared to be positive for the Gro a antigen, suggesting a phenotype frequency in the Dutch population of less than 0.01.