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M 9 + WINS Blood Group Phenotype: Further Observations
Author(s) -
Green Carole,
Daniels Geoff,
Skov Flemining,
Tippett Patricia
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb00316.x
Subject(s) - glycophorin , antigen , phenotype , microbiology and biotechnology , blood group antigens , antibody , erythrocyte membrane , chemistry , membrane , biology , immunology , biochemistry , gene
Red cells carrying the low‐frequency MNS antigen M 8 reacted with the only example of anti‐DANE, an antibody which had previously defined the GP.Dane (Mi.IX) phenotype. Furthermore, M g + cells reacted with the original anti‐Mur (serum of Mrs. Murrell), but with none of 14 other anti‐Mur. Therefore, M 8 + cells carry both DANE antigen and an atypical Mur antigen. Immunoblotting of membranes from M g + cells with anti‐M, and with eluates prepared from anti‐M g and Mrs. Murrell's serum demonstrated a glycophorin A (GPA) molecule whose mobility was increased by an apparent M r of about 3,000 presumably due to the loss of the three O‐glycans known to be absent from M g ‐active GPA.