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Complement Killing of Yersinia enterocolitica and Retention of the Bacteria by Leucocyte Removal Filters
Author(s) -
Gong J.,
Rawal B. D.,
Högman C. F.,
Vyas G. N.,
Nilsson B.,
Gustafsson I.
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb00304.x
Subject(s) - yersinia enterocolitica , microbiology and biotechnology , serotype , yersinia , yersinia infections , complement system , sepsis , complement (music) , alternative complement pathway , bacteria , biology , enterobacteriaceae , chemistry , immunology , antibody , escherichia coli , biochemistry , phenotype , genetics , complementation , gene
We report studies on the complement sensitivity of four strains of Yersinia enterocolitica , serotypes O:3, O:9, O:5,27, and O:20, isolated from blood units involved in transfusion fatalities. Complement in fresh CPD plasma killed Y. enterocolitica within 4 h at 22°C in 100% of the experiments. The bactericidal action was serotype and complement activation pathway dependent. Both classic and alternate pathways seemed to be active, but the latter to a lesser degree. When the classic pathway was blocked by chelation of Ca 2+ no complete killing was obtained. Complement did not enhance or condition Yersinia for leucocyte filter retention. Direct removal of Yersinia by filtration was also related to serotype; all strains were reduced by filtration in heat‐inactivated plasma, and all except serotype O:5,27 were reduced in Ca 2+ ‐chelated plasma. Our findings may explain why plasma products and platelet concentrates are rarely involved in Yersinia sepsis related to transfusion.