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A Simple Technique for Red Blood Cell Removal in Major ABO‐Incompatible Bone Marrow Transplantation
Author(s) -
Mayer Gerhard,
Wernet Dorothee,
Northoff Hinnak,
Schneider Werther
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb00291.x
Subject(s) - abo blood group system , buffy coat , bone marrow , transplantation , centrifugation , medicine , bone marrow transplantation , red blood cell , red cell , immunology , andrology , surgery , chemistry , chromatography
A simple technique for red blood cell (RBC) removal in major ABO‐incompatible bone marrow transplantation is reported requiring two centrifugation steps, special blood bags and a mechanical device to separate the buffy coat from RBCs within the bag. In 42 transplantations an average of 84% of nucleated cells was recovered with an average contamination of 7.5 ml packed RBCs. The preparations were well tolerated in all patients whose isoagglutinin titers had not been reduced. Bone marrow engraftment was not significantly different from control groups.