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Detection of HIV‐1 DNA in Leukocytes Using a Commercially Available Assay
Author(s) -
Zaaijer H. L.,
Cuypers H.T.M.,
Reesink H.W.,
Veen J. H.,
SchoonVisser S. C. E.,
Lelie P. N.
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb00282.x
Subject(s) - polymerase chain reaction , human immunodeficiency virus (hiv) , virology , medicine , dna , microbiology and biotechnology , biology , immunology , gene , genetics
Recently, an assay for detection of proviral HIV‐1 DNA in leukocytes became commercially available. This assay (Amplicor HIV‐1 test, Roche Diagnostic Systems) multiplies HIV‐1 DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV‐1‐infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false‐positive results, we advise to repeat initially reactive samples if no other data confirm HIV‐infection.