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Evidence for Expression of the Jo a Blood Group Antigen on the Gy a /Hy‐Active Glycoprotein
Author(s) -
PhD Frances A. Spring,
Reid Marion E.,
Nicholson Gordon
Publication year - 1994
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1994.tb00281.x
Subject(s) - antigen , microbiology and biotechnology , hemagglutination , glycoprotein , pronase , chemistry , trypsin , immune system , biology , biochemistry , immunology , enzyme
The phenotypic association between the non‐assigned high‐incidence antigen Jo a and the Gy a collection antigens Gy a and Hy was investigated by haemagglutination studies, flow cytometric analysis, immune precipitation and immunoblotting experiments. In haemagglutination tests anti‐Jo a gave the same pattern of reactivity with erythrocytes pre‐treated with pronase, trypsin, α‐chymotrypsin and thiol reducing agents as did anti‐Gy a and anti‐Hy. In addition, similar to that found for anti‐Gy a and anti‐Hy, anti Jo a also showed reduced binding, as determined by haemagglutination and flow cytometric analysis, to erythrocytes from patients with paroxysmal nocturnal haemoglobinuria. Immune precipitates prepared from radio‐iodinated antigen‐positive red cells with anti‐Jo a , anti‐Gy a and anti‐Hy gave similar results – a major component of M r 49,000–60,000 (the Gy a /Hy‐active glycoprotein) and a second component of M r 85,000–92,000 (this may be a dimer of the Gy a /Hy‐active glycoprotein, or a coprecipitated protein). These immune precipitates, when probed with both anti‐Gy a and anti‐Hy under non‐reducing conditions, gave a positive immunoblotting reaction to both the M r 49,000–60,000 and the M r 85,000–92,000 components. These results strongly suggest that the Jo a antigen is expressed on the same glycoprotein that carries the Gy a and Hy antigens.