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Plasma and Red‐Cell Glycolipid Patterns of Le(a+b+) and Le(a+b‐) Polynesians as Further Evidence of the Weak Secretor Gene Se w
Author(s) -
Henry S. M.,
Woodfield D. G.,
Samuelsson B. E.,
Oriol R.
Publication year - 1993
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1993.tb04527.x
Subject(s) - glycolipid , monoclonal antibody , microbiology and biotechnology , epitope , polyclonal antibodies , antigen , monoclonal , clone (java method) , biology , chemistry , b cell , antibody , gene , biochemistry , immunology
Monoclonal antibodies and thin‐layer chromatography were used to study the unusual erythrocyte Lewis phenotypes found in healthy Polynesians. A single monoclonal anti‐Le b reagent 073 (clone LM129) was found which could detect Le b antigen on the Polynesian erythrocytes of samples that were unreactive with various polyclonal and monoclonal anti‐Le b reagents. Glycolipid fractions prepared from the plasma and erythrocytes of selected Polynesian samples of red‐cell Le(a‐b‐), Le(a+b‐) and Le(a+b+) phenotypes were found to have Le b glycolipids. The Le b antigen in some individuals is so weakly expressed that it is undetectable by routine erythrocyte phenotyping. Unusually large glycolipids bearing the Le b epitope were also found in some Polynesian samples, although the contribution of these novel glycolipids to phenotyping is unclear. The inability to detect Le b by routine methods and the presence of novel structures can be partially explained in terms of the presence of a weak secretor gene Se w .