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Human Monoclonal Anti‐D‐Induced Clearance of Human D‐Positive Red Cells in a Chimpanzee Model
Author(s) -
Blancher A.,
Socha W. W.,
Roubinet F.,
Rowe A. W.,
Broly H.,
Byrne P.,
Holuigue M.,
Bouzidi A.,
Huart J. J.,
Ruffié J.
Publication year - 1993
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1993.tb04524.x
Subject(s) - monoclonal antibody , antibody , isotonic saline , in vivo , monoclonal , biology , immunology , microbiology and biotechnology , isotonic , chemistry , medicine , endocrinology
Two human monoclonal anti‐Rh 0 (D) antibodies, one IgG 1 , and one IgG 3 , were tested for their ability to clear human D‐positive red blood cells (RBCs) from chimpanzee circulation. Human RBCs (phenotype A 1 , R 1 r) from 1 donor were radiolabelled with chromium 51 and injected into 4 chimpanzees. One day later the control animal received isotonic saline whereas 2 animals received 400 μg of purified human monoclonal anti‐D, either IgG 1 or IgG 3 . The remaining animal received both antibodies together (200 μg of IgG 1 and 200 μg of IgG 3 ). Both individual antibody‐mediated clearance of human D‐positive cells and synergy was not observed when both antibodies were used in combination. IgG 1 was slightly more effective than IgG 3 . This animal model is a suitable alternative for conducting in vivo experiments in human beings, especially at the preclinical study phase of monoclonal anti‐D antibodies.