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Ultraviolet Irradiation Inhibits Killer‐Target Cell Interaction
Author(s) -
Kobata Tetsuji,
Ikeda Hisami,
Ohnishi Yoshiko,
Urushibara Noriko,
Nakata Sayuri Otsuka,
Takahashi Tsuneo A.,
Sekiguchi Sadayoshi
Publication year - 1993
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1993.tb04520.x
Subject(s) - ctl* , cytolysis , cytotoxic t cell , antigen , cell , cell adhesion , microbiology and biotechnology , cell–cell interaction , ultraviolet light , biology , cell culture , immunology , chemistry , in vitro , cd8 , biochemistry , genetics , photochemistry
The effects of ultraviolet (UV) irradiation on cell‐mediated cytolysis were examined in order to clarify the inhibitory mechanisms of allosensitization by UV irradiation. UV‐B‐irradiated target cells (Sa; an Epstein‐Barr virus‐transformed B cell line) exhibited more resistance against alloreactive cytotoxic T lymphocytes (CTL) than mitomycin C (MMC)‐treated target cells. In the conjugate formation assay, UV‐B‐irradiated target cells showed a considerably lower binding to alloreactive CTL than MMC‐treated target cells. UV‐B irradiation induced a reduction of HLA‐class I, ‐DR, CD54 (ICAM‐1) and CD58 (LFA‐3) expression on target cells. However, it does not seem to contribute to the inhibition of cell adhesion induced by UV‐B irradiation because a similar reduction of cell surface antigens was observed in MMC‐treated target cells. Number of cells capped with anti‐HLA‐class I, ‐DR, CD54 or CD58 monoclonal antibody were markedly reduced by UV‐B irradiation compared to that by MMC treatment. These findings suggest the possibility that the inhibition of cell adhesion between UV‐B‐irradiated Sa target cells and alloreactive CTL is due to the impaired mobility of cell surface antigens which will affect the early process of cell‐mediated cytolysis.