Premium
Blood Donations Indeterminate in HIV‐1 Western Blot Analysed by IgM Immunoblot and Polymerase Chain Reaction
Author(s) -
Hopf H. Knüver,
Heinze H.,
Lambrecht B.,
Mohr H.,
Beyer J.,
Schmitt H.
Publication year - 1993
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1993.tb02524.x
Subject(s) - polymerase chain reaction , indeterminate , virology , antibody , blood donor , western blot , southern blot , microbiology and biotechnology , biology , viral disease , serology , virus , immunology , medicine , dna , gene , genetics , mathematics , pure mathematics
The presence of IgM antibodies to human immunodeficiency virus 1 (HIV‐1) was investigated in blood donor sera which were indeterminate in anti‐HIV‐1 IgG Western blot testing. In 7 of 173 instances out of approximately 1,000,000 blood donation sera with an isolated anti‐p24 IgG produced an anti‐gp41‐45 IgM immunoblot reaction. Applying polymerase chain reaction (PCR) to 29 indeterminate samples out of approximately 125,000 blood donations it was found that 2 of them were IgM‐positive and also contained HIV‐1‐specific DNA sequences. Eleven months later 1 of these 2 donors was retested and found IgM and PCR negative.