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Detection of Established and Novel Alleles of the HLA‐DPB1 Locus by PCR‐SSO
Author(s) -
Pickl Winfried F.,
Faé Ingrid,
Fischer Gottfried F.
Publication year - 1993
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1993.tb02173.x
Subject(s) - locus (genetics) , allele , genetics , biology , human leukocyte antigen , polymerase chain reaction , genotype , microbiology and biotechnology , gene , oligonucleotide , antigen
The polymorphism at the HLA‐DPB1 locus was investigated in 490 Austrian individuals. Exon 2 sequences of the HLA‐DPB1 genes were amplified by polymerase chain reaction and probed with a panel of sequence‐specific oligonucleotide probes. HLA‐DPB1 genotypes were deduced from the hybridization pattern of the probes. HLA‐DPB1 allele frequencies and Hardy‐Weinberg equilibrium were calculated. Linkage disequilibria between HLA‐DPB1 and HLA‐DQA1 alleles were determined. Four novel hybridization patterns indicating new alleles were found.