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Preparation of Intermediate‐Purity Factor VIII Concentrate by Direct Gel Filtration of Cryoprecipitate
Author(s) -
Teh L. C.
Publication year - 1993
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1993.tb02164.x
Subject(s) - cryoprecipitate , chemistry , chromatography , size exclusion chromatography , yield (engineering) , solvent , filtration (mathematics) , blood product , biochemistry , materials science , fibrinogen , surgery , enzyme , medicine , statistics , mathematics , metallurgy
Abstract We report a new method to produce a solvent/detergent‐treated and severe dry heat‐treated factor VIII (FVIII) concentrate (3‐6IU FVIII:C/mg protein). This method, which uses a single purification step after cryoprecipitation, is suitable for scale‐up to production levels. FVIII was obtained from solvent/detergent‐treated cryoprecipitate by a single gel filtration step using Sephacryl S‐400HR. The freeze‐dried product was stable to heating at 80°C for 72 h. The yield of the solvent/detergent and severe dry heat‐treated product was 230 IU FVIII: C/kg plasma. The reconstituted product gave a 10% loss in FVIII: C activity after heating at 37°C for 6 h. The feasibility of this method suggests that gel filtration using S‐400HR can be used solely or as part of a purification process for the preparation of high‐purity FVIII concentrates.