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Direct Agglutination of Weak D Red Cells by Tetramolecular Complexes Containing Monoclonal IgG Anti‐D
Author(s) -
Lemieux Réal,
Verrette Sylvain,
Broly Hervé,
Perron Serge
Publication year - 1993
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1993.tb02131.x
Subject(s) - avidity , monoclonal antibody , agglutination (biology) , isotype , antibody , serology , red blood cell , microbiology and biotechnology , monoclonal , subclass , immunology , chemistry , red cell , virology , biology , medicine
The capacity of blood group antibodies to agglutinate red cells suspended in saline is largely dependent on the antibody isotype. The immunological cross‐linking of IgG antibodies has previously been described as a means to increase the reactivity of IgG in many situations. We have prepared anti‐D‐containing complexes by blending a human IgG anti‐D monoclonal antibody (mAb) and a murine anti‐human IgG mAb. In standard red cell serology assays, the anti‐D complexes exhibited a very high avidity and could agglutinate weak D‐positive red cells in direct saline testing. These results indicate that potent saline hemagglutinating reagents of RhD and eventually of other blood group specificities can be prepared from IgG mAbs.