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Studies in Red Blood Cell Preservation
Author(s) -
Greenwalt T.J.,
Dumaswala U.J.,
Dhingra N.,
Allen C.M.,
Silberstein E.B.
Publication year - 1993
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1993.tb02122.x
Subject(s) - mannitol , blood preservation , red blood cell , shelf life , chemistry , cryopreservation , andrology , medicine , chromatography , biochemistry , food science , biology , embryo , microbiology and biotechnology
Studies were conducted to examine whether an experimental additive solution (EAS‐2) containing, in m M : 20 NH 4 Cl, 30 Na 2 HPO 4 , 2 adenine, 110 dextrose, 55 mannitol, pH 7.15, would be useful to extend the storage shelf life of human RBCs. With 6 pairs of split units, ATP concentrations were better maintained for 12 weeks with EAS‐2 than with Adsol® (1.8 vs. 1.1 μmol/gHb, respectively, p = 0.002). Autologous 24‐hours 51 Cr viability values for split units in the same donors were: on 6 paired units at 8 weeks, EAS‐2 87.0±4.5%, Adsol 72.6±2.3%, p = 0.004; on 11 paired units at 9 weeks, EAS‐2 79.5±7.1%, Adsol 68.2±10.1%, p = 0.0003. The data suggest that packed RBCs stored for 9 weeks with EAS‐2 will be suitable for transfusion following the removal of supernatant with a single washing step.