Premium
Diversity of Human Anti‐D Monoclonal Antibodies Revealed by Reactions with Chimpanzee Red Blood Cells
Author(s) -
Blancher A.,
Socha W. W.,
Ruffie J.
Publication year - 1992
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1992.tb02496.x
Subject(s) - monoclonal antibody , epitope , antibody , biology , antigen , microbiology and biotechnology , agglutination (biology) , primate , human blood , phenotype , monoclonal , virology , immunology , genetics , gene , physiology , neuroscience
Fifty‐three human anti‐D monoclonal antibodies (mAbs) revealed a striking diversity of reactions in tests with panels of chimpanzee red blood cells (RBCs) of various R‐C‐E‐F blood group phenotypes (counterparts of the human Rh‐Hr groups). The reactivities of these antibodies, which depended on the agglutination technique used, could be classified into four main types. These patterns of reactivity of anti‐D mAbs with chimpanzee RBCs showed only limited correlation with types of reactions observed with human D variant RBCs. Primate red cells may, therefore, constitute an independent test system for subclassification of human monoclonal antibodies. Comparison of reactivities of human anti‐D mAbs with chimpanzee and human D variant RBCs confirms the homology between the chimpanzee R c , and the human D antigens. The chimpanzee R c shares with human D the epitopes epD5, epD6/7 and epD8, but lacks epitopes epD1, epD2, epD3 and epD4 of the Rh mosaic, thus resembling the human D variants IVb and Vc.