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Studies in Red Blood Cell Preservation
Author(s) -
Dumaswala U.J.,
Petrosky T.L.,
Greenwalt T.J.
Publication year - 1992
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1992.tb01212.x
Subject(s) - echinocyte , inosine , erythrocyte fragility , red blood cell , hemoglobin , incubation , saline , tonicity , vesicle , mean corpuscular volume , andrology , phosphate buffered saline , chemistry , mean corpuscular hemoglobin concentration , osmotic concentration , hypertonic saline , biology , biochemistry , chromatography , immunology , membrane , endocrinology , medicine , hemolysis , enzyme
The purpose of this study was to examine whether vesiculation of RBC plays a significant role in their rejuvenation. Outdated units of Adsol® blood, were divided into two aliquots and incubated with equal volumes of a solution of 100 m M pyruvate and inosine, 103 m M phosphate and 5 m M adenine (PIPA) or 0.9% saline. Following 1 h incubation, vesicles were isolated from the supernatants and quantitated for hemoglobin content. Restoration of RBC ATP, 2,3‐DPG, morphology, and osmotic fragility after rejuvenation was satisfactory. The postrejuvenation mean corpuscular volumes (88.2+6.9 fl) were significantly lower (p<0.001) than the prerejuvenation (94.6+6.8 fl) and control (104.0+7.3 fl) volumes. The hemoglobin shed in vesicles during rejuvenation was significantly greater than in the saline controls (0.44+0.31 vs. 0.18+0.10 mg/dl RBCs; p = 0.026). These data suggest that the decreased MCV following rejuvenation is in part due to membrane loss in exocytic vesiculation.