Premium
Serological and Chemical Specificities of Twelve Monoclonal Anti‐Le a and Anti‐Le b Antibodies
Author(s) -
Good A. H.,
Yau O.,
Lamontagne L. R.,
Oriol R.
Publication year - 1992
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1992.tb01195.x
Subject(s) - chemistry , stereochemistry , antibody , reactivity (psychology) , monoclonal antibody , serology , microbiology and biotechnology , biology , immunology , medicine , alternative medicine , pathology
The serological specificities of twelve hybridomas were compared as to their chemical reactivity as determined using direct binding to synthetic carbohydrate structures. All anti‐Le a cross‐react with type‐1‐precursor structures and three different variants of anti‐Le a could be defined by their binding to type‐3‐precursor chains, sialylated compounds and the monosaccharide D‐ galactose. Three major reactivity patterns were also identified among anti‐Le b reagents. Anti‐Le bL cross‐react with Le a and do not significantly bind to H‐related structures. Anti‐Le bH L had both anti‐Le bL ‐like activity (cross‐reaction with Le a ) and anti‐Le bH ‐like activity (cross‐reaction with H). Finally, anti‐Le bH cross‐reacts strongly with H compounds and do not bind to Le a . The binding pattern of anti‐Le bL suggests that these antibodies have lower affinity for ALe b and BLe b pentasaccharides than anti‐Le bH . All these specificities are not absolute, but rather are expressed as members of a quantitative progressive varying series, sugesting the existence of a whole range of antibody specificities gradually changing from Le a → Le a, b → Le bL → Le bH, L → Le bH . The results suggest that anti‐Le bL will always cross‐react with Le a and that anti‐Le bH will always cross‐react with H related structures. However, under certain well‐defined conditions these cross‐reactions may not be apparent and antibodies might behave as specific anti‐Le a or anti‐Le b in certain tests.