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Analysis of the Gene Polymorphism of ABO Blood Group Specific Transferases Helps Diagnosis of Acquired B Status
Author(s) -
Fischer G. F.,
Faé Ingrid,
Dub Elisabeth,
Pickl W. F.
Publication year - 1992
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1992.tb01181.x
Subject(s) - abo blood group system , polymerase chain reaction , biology , gene , glycosyltransferase , transferase , genetics , microbiology and biotechnology , restriction fragment length polymorphism , cleaved amplified polymorphic sequence , polymorphism (computer science) , group a , genotype , enzyme , biochemistry , medicine
Blood group typing of an aged patient suffering from ileus provided evidence for an acquired B. As a parameter independent of cell membrane molecules or secreted blood group substances, the nucleotide sequence polymorphism of A and B transferases was investigated. Restriction fragment length polymorphism of DNA chains amplified in a polymerase chain reaction from the coding region of the glycosyltransferase indicated that no gene for B transferase was present in the patient's genome. We conclude that the assessment of polymorphism of ABO blood group transferase can be used as a marker independent of blood group molecules for confirming a suspected acquired B.