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A Human Anti‐Jk b Monoclonal Antibody
Author(s) -
LecointreCoatmelec M.,
Bourel D.,
Ferrette J.,
Genetet B.
Publication year - 1991
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1991.tb00956.x
Subject(s) - monoclonal antibody , peripheral blood mononuclear cell , antibody , virology , microbiology and biotechnology , monoclonal , cloning (programming) , chemistry , immunology , biology , biochemistry , in vitro , computer science , programming language
. Peripheral blood mononuclear cells of an immunized patient were transformed with Epstein‐Barr virus and then fused with P 3 X 63 Ag 8 mouse myeloma cells by polyethylene glycol. After the cloning, a hybridoma cell line secreting specific anti‐Jk b monoclonal antibody was isolated. The antibody was produced in supernatant form and tested for its use as a blood grouping reagent.