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Enhanced Detection of HIV‐1 Sequences Using Polymerase Chain Reaction and a Liquid Hybridization Technique
Author(s) -
Bruisten S. M.,
Koppelman M. H. G. M.,
Poel C. L.,
Huisman J. G.
Publication year - 1991
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1991.tb00922.x
Subject(s) - polymerase chain reaction , indeterminate , microbiology and biotechnology , human immunodeficiency virus (hiv) , blot , serology , polymerase , nucleic acid thermodynamics , biology , virology , dna , antibody , gene , immunology , genetics , base sequence , mathematics , pure mathematics
Abstract. In this report we describe a sensitive HIV‐1 detection method which is applicable for confirmation testing of donors whose blood sample gives indeterminate viral‐serology results. The method involves performing a polymerase chain reaction (PCR) and detecting the generated fragments using liquid hybridization and gel retardation. We found that it is as specific as blotting on a filter and hybridization with an internal probe but at least tenfold more sensitive. After applying it on DNA samples of a panel of 11 persistent indeterminate anti‐p24 gag ‐reactive donors, none was found to be PCR positive. Considering other negative virological and biochemical test results and case‐historical data, these donors are not likely to be HIV‐1 infected.