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Identification by Immunoblotting of the Erythrocyte Membrane Sialoglycoproteins That Carry the Vw and Mur Antigens
Author(s) -
Herron Bob,
Smith Graham A.
Publication year - 1991
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1991.tb00884.x
Subject(s) - sialoglycoproteins , neuraminidase , sialoglycoprotein , microbiology and biotechnology , monoclonal antibody , antigen , staining , hemagglutination , biology , chemistry , antibody , glycoprotein , virology , immunology , virus , genetics
. Immunoblotting of the separated membrane components of MiI erythrocytes with anti‐Vw identified a band of M r 40,000 with a mobility close to that of β‐sialoglycoprotein corresponding to the abnormal α‐sialoglycoprotein present in MiI cells. A comparison of results obtained when MiIII erythrocytes were immunoblotted with anti‐Mur, anti‐s and the monoclonal antibody R1.3, indicated that the Mur antigen is located on the abnormal δ‐sialoglycoprotein of M r 36,000 in MiIII erythrocytes. Prior treatment of MiI erythrocytes with neuraminidase resulted in an increase in the intensity of staining of the anti‐Vw reactive component. This was consistent with the enhanced reactions observed in haemagglutination tests with neuraminidase‐treated erythrocytes. The mobility of the Vw component was reduced when erythrocytes were pre‐treated with low concentrations of neuraminidase but increased when higher concentrations of neuraminidase were used.