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Refrigerated Storage of Washed Red Cells 1
Author(s) -
Meryman H. T.,
Hornblower M.,
Keegan T.,
Syring R.,
Heaton A.,
MesbahKarimi N.,
Bross J.
Publication year - 1991
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1991.tb00880.x
Subject(s) - intracellular , hemolysis , chemistry , adenosine triphosphate , phosphate , intracellular ph , washout , red cell , biochemistry , osmotic concentration , glycolysis , biophysics , in vivo , phosphate buffered saline , metabolism , chromatography , biology , medicine , immunology , microbiology and biotechnology
. Red cells washed and stored in a citrate‐phosphate‐glucose‐adenine solution at pH 7.4–7.6 demonstrate excellent maintenance of adenosine triphosphate, elevation of 2,3‐diphosphoglycerate well above normal levels for more than 6 weeks, reduced hemolysis and 24‐hour in vivo survival comparable to that of cells stored in ADSOL. These results can be attributed in part to a chloride shift in which the washout of intracellular chloride is associated with an influx of OH“, which increases intracellular pH and thereby increases the rate of glycolysis. The phosphate functions primarily as a buffer to maintain both extra‐ and intracellular pH. Reducing the effective osmolality of the storage solution reduces hemolysis and improves cell morphology.