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Miltenberger Class IX of the MNS Blood Group System
Author(s) -
Skov Flemming,
Green Carole,
Daniels Geoff,
Khalid Ghizala,
Tippett Patricia
Publication year - 1991
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1991.tb00258.x
Subject(s) - glycophorin , trypsin , cleavage (geology) , chymotrypsin , monoclonal antibody , amino acid , chemistry , residue (chemistry) , antibody , glycosylation , serology , microbiology and biotechnology , biochemistry , antigen , biology , immunology , enzyme , paleontology , fracture (geology)
Mi.IX is a new phenotype in the Miltenberger series of the MNS blood group system with a frequency of 0.43% in Denmark. Mi.IX red cells are Mur+ but do not express any of the other established Miltenberger determinants. They react with a new antibody, anti‐DANE, which defines a determinant present on Mi.IX cells but not on cells of other Miltenberger phenotypes. Four Mi.IX propositi have been found. Their families show that Mi IX is inherited with a MS complex (lod score 3.69 at θ = 0.00) which produces a trypsin‐resistant M antigen. DANE has been allotted the ISBT number 002032 (MNS32). Serological and immunochemical studies with human and monoclonal antibodies to various determinants on glycophorin A (GPA) suggest that Mi.IX is associated with an aberrant GPA molecule that lacks the trypsin cleavage site at amino‐acid residue 39, retains the chymotrypsin cleavage site at residue 34 and has an apparent M r of about 1,000 less than normal GPA. It is proposed that this Mi.IX molecule has an amino acid and possibly also a glycosylation change in the region of amino‐acid residues 35–39.