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Monoclonal Antibody to Blood Group Glycosyltransferases, Produced by Hybrids Constructed with Epstein‐Barr‐Virus‐Transformed B Lymphocytes from a Patient with ABO‐Incompatible Bone Marrow Transplant and Mouse Myeloma Cells
Author(s) -
Kominato Yoshihiko,
Fujikura Takashi,
Shimada Ichirou,
Takizawa Hisao,
Hayashi Kyoko,
Mori Takao,
Matsuda Tamotsu
Publication year - 1990
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1990.tb05022.x
Subject(s) - abo blood group system , antibody , glycosyltransferase , monoclonal antibody , bone marrow , biology , virology , virus , microbiology and biotechnology , transplantation , immunology , medicine , enzyme , biochemistry
. A patient with chronic myeloid leukemia secreted an antibody to blood group glycosyltransferases after ABO‐incompatible bone marrow transplantation (B recipient/O donor). Peripheral B lymphocytes from the recipient were transformed with Epstein‐Barr virus, and then fused by polyethylene glycol with mouse myeloma cell line P3‐X63/Ag8.653. After the cloning of the hybridoma cells, a cell line which produced human IgM antibody to blood group glycosyltransferases was established. The antibody completely neutralized B transferase activity at low concentration, while a larger amount of immunoglobulins was required to neutralize A transferase activity.