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Erythrocyte Antibody Screening in Solid‐Phase: A Comparison of Two Solid‐Phase Microplate Assays with the Indirect Antiglobulin Test in Polyethylene Glycol for the Detection of Irregular Erythrocyte Antibodies
Author(s) -
Hazenberg Corinne A.M.,
Mulder Margriet B.,
Beelen Josée M.
Publication year - 1990
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1990.tb05018.x
Subject(s) - antibody , chemistry , coombs test , chromatography , sensitization , polyethylene glycol , peg ratio , immunology , medicine , biochemistry , finance , economics
. The screening of a serum for irregular erythrocyte antibodies in the indirect antiglobulin test is a well‐established technique. We compared the test results of two different solid‐phase microplate indirect antiglobulin tests with a liquid‐phase indirect antiglobulin test in tubes. Antibody screening with both solid‐phase microplate techniques proved to be more sensitive than the liquid‐phase indirect antiglobulin test. In addition, a difference in sensitivity between the two solid‐phase techniques was observed: prior immobilization of test erythrocytes on the microplate followed by incubation with a serum and detection of sensitization with antihuman IgG‐coated detector cells gave better test results than secondary immobilization on the microplate of test erythrocytes sensitized with antibodies and an antihuman globulin serum.