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Correlation of Monocyte‐Monolayer Assay Results, Number of Erythrocyte‐Bound IgG Molecules, and IgG Subclass Composition in the Study of Red Cell Alloantibodies Other Than D
Author(s) -
Zupańska B.,
Brojer E.,
McIntosh J.,
Seyfried H.,
Howell P.
Publication year - 1990
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1990.tb04999.x
Subject(s) - subclass , immunology , chemistry , antibody , medicine
. Comparisons have been made between the serological and immunological characteristics of 42 blood group allonantibodies (other than D) covering twelve systems using a monocyte‐monolayer assay (MMA), a radiometric antiglobulin test for antibody binding and IgG subclass determinations. The results of the MMA correlated well with the level of IgG molecules bound on incompatible cells, and the highest levels in both cases were associated with the presence of the IgG3 subclass. However, limited clinical data shows that, while in general the MMA clearly identifies the clinically significant antibodies, the correlation with the degree of clinical outcome is less well defined, and in some instances other factors may be operating to ameliorate the in vivo effect of the antibody.