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A Simple Plasma Anticoagulant‐Exchange Method to Increase the Recovery of Factor VIII in Therapeutic Concentrates
Author(s) -
Cumming A. M.,
Wensley R. T.,
Winkelman L.,
Lane R. S.
Publication year - 1990
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1990.tb04997.x
Subject(s) - plasmapheresis , heparin , anticoagulant , chemistry , chromatography , calcium , surgery , medicine , biochemistry , immunology , antibody , organic chemistry
. Donor blood, primarily anticoagulated by acid citrate dextrose formula A (ACD‐A), was separated by means of the HemaScience Autopheresis C TM plasmapheresis device. The citrated plasma was collected directly into a solution of heparin and calcium chloride to achieve a final plasma‐ionised calcium concentration of approximately 2 m M , and a heparin concentration of 1.0 IU/ml. Heparin at this concentration provided adequate anticoagulation, and did not result in insoluble cryoprecipitates. Three pairs of donor‐matched 4‐kg plasma pools (anticoagulant‐exchanged variant and ACD‐A‐anticoagulated control) were constructed and subsequently fractionated to an intermediate stage. The mean recovery of factor VIII from 3 anticoagulant‐exchanged pools (394 IU/kg) was 23% greater than the mean recovery from the matched control pools (319 IU/kg). This increased recovery was not achieved at the expense of specific activity.