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A New Type of Blood Component Collector: Plasma Separation Using Gravity without any Electrical Devices
Author(s) -
Sekiguchi Sadayoshi,
Takahashi Tsuneo A.,
Yamamoto Sadamitsu,
Hasegawa Hideya,
Takenaka Yoshinori,
Suemitsu Junsuke,
Fukumi Hirokazu
Publication year - 1990
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1990.tb02087.x
Subject(s) - separator (oil production) , chromatography , plasma , chemistry , albumin , hemolysis , partial thromboplastin time , platelet , red cell , blood proteins , whole blood , fresh frozen plasma , materials science , immunology , biochemistry , medicine , physics , quantum mechanics , thermodynamics
. A new type of blood component collector (BCC) was developed to divide 450 ml of whole blood into plasma and a red cell concentrate using gravity without electrical devices. This BCC system is composed of one whole blood collection bag, two product collection bags and a plasma separator, which consists of a bundle of hydrophilized polyethylene hollow fibers (0.2 μm pore size). Without rinsing the plasma separator, the whole blood (458.1±13.5 ml, n = 22) was run through the separator using gravity without a pump. An average of 175.9 ml of plasma was collected within 11 min without hemolysis. In this completely cell‐free plasma, the recovery of total protein, albumin, globulin, IgG and IgA was nearly 100%. Prothrombin time and activated partial thromboplastin time were in a normal range and the activity of coagulation factors did not change after the separation. In the red cell concentrate, the recovery of red cells, white cells and platelets was 94.7, 98.4 and 82.7%, respectively. Osmotic fragility of red cells, platelet morphology and functions did not change. These observations suggest that this new type of BCC is useful as a simple, fast and safe component collector.