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Human Monoclonal Anti‐D with Reactivity against Category D VI Cells Used in Blood Grouping and Determination of the Incidence of the Category D VI Phenotype in the D U Population
Author(s) -
Leader K. A.,
Kumpel B. M.,
Poole G. D.,
Kirkwood J. T.,
Merry A. H.,
Bradley B. A.
Publication year - 1990
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1990.tb02071.x
Subject(s) - monoclonal antibody , microbiology and biotechnology , antibody , red blood cell , phenotype , monoclonal , biology , immunology , genetics , gene
. B‐lymphoblastoid cell lines transformed by Epstein‐Barr virus were produced from cells obtained from a hyperimmunised donor with serum anti‐D activity against category D VI red cells and enriched for this activity by rosetting with category D VI red cells. Three clones produced IgGl anti‐D and had stable cell growth and continuous secretion of antibody in prolonged culture. The monoclonal antibodies reacted with category D VI red cells, when assessed manually and in an automated blood grouping system, and are useful blood grouping reagents for the detection of the category D VI phenotype. Using a radiometric technique, the number of antibody molecules bound to category D VI red cells from 5 individuals was estimated to range from 2,800 to 11,200 per cell. Five percent of blood donors classed as D u in the south western region were found to have the category D VI phenotype.