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High Molecular Weight Aggregate Content of Heated and Unheated Factor VIII Products Determined by Fast‐Protein Liquid Chromatography
Author(s) -
Dawes J.,
Freeman L.,
Dawson N. J.,
Pepper D. S.,
Barrowcliffe T. W.
Publication year - 1990
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1990.tb02051.x
Subject(s) - chemistry , chromatography , fibrinogen , molar mass distribution , aggregate (composite) , biochemistry , materials science , organic chemistry , polymer , composite material
. The molecular‐weight distribution of proteins in factor VIII concentrates has been analysed by fast‐protein liquid chromatography. The proportion of high‐molecular‐weight (HMW) aggregates in one product increased on freeze‐drying and heating, with fibrinogen and fibronectin being the main protein components of the HMW peak. In all other concentrates, the HMW peak was less than or equal to 5% of the total protein content and there were no differences in HMW content according to purity or method of viral inactivation.