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Effects of Hypertonic Saline (7.5%)/Dextran 70 on Human Red Cell Typing, Lysis, and Metabolism in vitro 1
Author(s) -
Moore Gerald L.,
Summary James J.,
Dubick Michael A.,
Ledford Mary E.,
Ryan Barbara A.,
Gonzales Armando,
Wade Charles E.
Publication year - 1990
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1990.tb00242.x
Subject(s) - lysis , in vitro , tonicity , dextran , red cell , red blood cell , hypertonic saline , metabolism , chemistry , saline , biochemistry , biology , medicine , endocrinology
The introduction of a 7.5% hypertonic saline/6% dextran 70 (HSD) solution into clinical trials for the treatment of hypovolemic states, and the past concerns regarding the possible interference of dextran with blood serology, prompted us to investigate the effects of HSD on human red‐cell typing and stability. HSD was evaluated with fresh and 35‐day stored CPDA‐1 red cells from 12 healthy donors. A 1:5 mixture of HSD to blood in vitro had no effect on ABO, Rh, and MN typing in both fresh and stored blood. HSD produced no significant lysis with fresh cells and a minimal level with stored blood. No evidence of metabolic or morphologic changes was seen after HSD treatment. The results of this study suggest that the clinical use of HSD for the treatment of hemorrhagic shock will not affect blood group determinations or red‐cell stability from stored blood which may be infused after the HSD‐treated patient is transported to a hospital.