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Absence of HIV DNA Sequences in Seronegative Polytransfused Thalassemic Patients
Author(s) -
Lefrère JeanJacques,
Montalembert Mariane,
Mariotti Martine,
Girot Robert,
Salmon Charles,
Rouger Philippe,
Rey Jean
Publication year - 1990
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1990.tb00240.x
Subject(s) - seroconversion , polymerase chain reaction , virology , primer (cosmetics) , human immunodeficiency virus (hiv) , antibody , medicine , biology , immunology , genetics , gene , chemistry , organic chemistry
The risk of infection with human immunodeficiency virus (HIV) by transfusion is not totally eliminated, since contaminated blood given before seroconversion to HIV is not detected on the actual biological screening. We used the polymerase chain reaction (PCR) assay (with one primer pair in the gag region and two in the pol region) to detect HIV DNA sequences in 30 seronegative polytransfused thalassemic patients and in 60 seropositive individuals (used as positive controls). We did not observe PCR‐positive HIV‐antibody‐negative results in seronegative polytransfused patients.