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Coexpression of Sialosyl‐Tn (NeuAcα2→6GalNAcαl→0‐Ser/Thr) and Tn (GalNAcα1→O‐Ser/Thr) Blood Group Antigens on Tn Erythrocytes 1
Author(s) -
Kjeldsen T.,
Hakomori S.,
Springer G. F.,
Desai P.,
Harris T.,
Clausen H.
Publication year - 1989
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1989.tb04990.x
Subject(s) - polyclonal antibodies , antigen , monoclonal antibody , microbiology and biotechnology , antiserum , antibody , biology , agglutination (biology) , red blood cell , chemistry , biochemistry , immunology
Abstract. Polyagglutinable erythrocytes expressing the rare human blood group phenotype Tn were tested for expression of both Tn (GalNAcα1→O‐Ser/Thr) and sialosyl‐Tn (NeuAcα2→6GalNAcα1→O‐Ser/Thr) antigens by agglutination with a panel of well‐characterized monoclonal antibodies specifically directed to Tn and sialosyl‐Tn antigens, respectively. Tn erythrocytes from 4 patients were strongly agglutinated by both sialosyl‐Tn and Tn‐specific monoclonal antibodies, indicating coexpression of sialosyl‐Tn and Tn antigens on the cell membrane surface of Tn polyagglutinable erythrocytes. Human polyclonal anti‐Tn antisera were found to express weak and variable anti‐sialosyl‐Tn antibodies in conjunction with the anti‐Tn antibodies. The Tn phenotype thus involves not only the classically known Tn antigens but also sialosyl‐Tn antigens. However, the human anti‐Tn antibodies may be directed mainly toward the Tn antigen (GalNAcα1→O‐Ser/Thr).