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Immunoglobulin G Subclass Distribution in Three Human Intravenous Immunoglobulin Preparations
Author(s) -
Burckhardt Johann J.,
Gardi Andreas,
Oxelius ViviAnne,
Preud'homme JeanLouis,
Scherz Rudolf,
Skvaril Frantisek,
Heiniger HansJörg
Publication year - 1989
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1989.tb04976.x
Subject(s) - subclass , radial immunodiffusion , polyclonal antibodies , antibody , immunology , immunoglobulin g , immunoglobulin e , medicine , isotype , monoclonal antibody
. In immunodeficiency patients the lack of immunoglobulins (Ig) can be total or partial with a specific IgG subclass imbalance masked by normal values for total IgG. In the latter case therapy with intravenous IgG preparations (IVIG) is generally beneficial, provided the IVIG preparations used originate from large pools of normal blood donors and exhibit a normal IgG subclass distribution. We have analyzed the subclass distribution of three IVIG products: Sandoglobulin® (SAGL), Gamimune®N (GI), Gammagard® (GG), 6–10 lots each, in four different laboratories. The competitive enzyme immunoassays and radial immunodiffusion methods used different monoclonal and polyclonal antibodies specific for IgG 1 , IgG 2 , IgG 3 , and IgG 4 , respectively. Despite minor interlaboratory differences, the results show that the slightly lower IgG 1 content of SAGL versus GI and GG was quantitatively compensated by a higher proportion of IgG 2 , that no differences existed in IgG 3 levels, but that one preparation (SAGL) contained 2–3% of IgG 4 compared to 0.5–1.5% in GI and below 0.5% in GG. This difference was significant, the two latter preparations being at or below the lower limit of what are considered to be normal values found in human adults. Such differences may have important clinical consequences.