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Ability of Monoclonal Anti‐D Antibodies to Promote the Binding of Red Cells to Lymphocytes, Granulocytes and Monocytes
Author(s) -
Merry A.H.,
Brojer E.,
Zupanska B.,
Hadley A.G.,
Kumpel B.M.,
HughesJones N.C.
Publication year - 1989
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1989.tb03048.x
Subject(s) - polyclonal antibodies , monoclonal antibody , rosette (schizont appearance) , antiserum , monoclonal , antibody , microbiology and biotechnology , biology , monocyte , receptor , immunology , biochemistry
Abstract. Red cells sensitized with IgG1 or IgG3 monoclonal anti‐D antibodies were used in rosette assays with human lymphocytes, monocytes and granulocytes. With all three cell types, IgG3 antibodies promoted a greater degree of rosette formation than IgG1 antibodies. Monocytes required a minimum of about 0.5 times 10 3 IgG3 molecules per red cell for rosette formation, and granulocytes and lymphocytes required around 1 times 10 4 IgG3 molecules per red cell. Approximately 80% of monocytes and granulocytes and 10% of lymphocytes were capable of rosette formation. These results are consistent with differences in the number and affinity of Fc receptors on different leucocytes. When compared with previous data these results suggest that binding of monocytes to monoclonal anti‐D sensitized red cells is very similar to that of red cells sensitized with polyclonal antisera. Lymphocytes and granulocytes, however, appear to bind less well to red cells sensitized with certain monoclonal antibodies than with polyclonal antibodies. These findings may be of relevance to the prophylactic use of monoclonal anti‐D antibodies.