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Peroxidative Events in Stored Platelet Concentrates 1
Author(s) -
Fagiolo E.,
Lippa S.,
Mores N.,
Oradei A.,
Aureli V.
Publication year - 1989
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1989.tb03043.x
Subject(s) - lactic acid , platelet , chemistry , coenzyme q10 , vitamin e , biochemistry , lipid peroxidation , bicarbonate , lactic acidosis , lactate dehydrogenase , cofactor , antioxidant , lactic dehydrogenase , enzyme , medicine , biology , bacteria , genetics , organic chemistry
. Changes in metabolic and functional activity of platelets stored as platelet concentrates in plastic bags highly permeable to gases were investigated. The following parameters were measured daily: pH, pO 2 , pCO 2 , HCO 3 , glucose, lactic acid, lactic dehydrogenase, cellular ATP and platelet aggregation induced by different agents (collagen and ADP). As indexes of lipid peroxidative damage, the cellular levels of conjugated dienes, malonyldialdehyde and some antioxidant molecules such as coenzyme Q10 and vitamin E were determined. A marked increase in pO 2 , conjugated dienes, malonyldialdehyde, lactic acid and lactic dehydrogenase activity was observed during the preservation. Platelet ATP content was unmodified and a remarkable decrease in platelet aggregability was found. pCO 2 , cyclooxygenase activity, vitamin E, coenzyme Q10, bicarbonate and glucose showed a rapid fall. Our data seem to indicate a preservation of platelet metabolic activity and a correlation between increased lipid peroxidation and functional impairement.