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Testing Efficacy of Anti‐D Sera by a Panel of Donor Red Cells with Weak Reacting D Antigen and with Partial D Antigens
Author(s) -
Rhenen D.J.,
Thijssen P.M.H.J.,
Overbeeke M.A.M.
Publication year - 1989
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1989.tb02041.x
Subject(s) - antigen , polyclonal antibodies , monoclonal , antibody , monoclonal antibody , immunology , negative control , microbiology and biotechnology , medicine , chemistry , biology , traditional medicine
. In 1987 the definition of an Rh‐negative donor in the Netherlands was changed from C‐, E‐ as well as D u ‐negative to D‐negative only. The use of 2 different strong anti‐D sera without an antiglobulin phase (D u test) was considered sufficient to reveal the clinically important D antigen. In applying this policy, we identified 32 donors in 13,500 consecutive blood donations whose indirect antiglobulin test (IAT) (D u )‐positive red cells gave negative reactions with at least 2 out of 11 anti‐D sera and whose cells might therefore be typed as Rh(‐D‐)‐negative in routine investigations. IgG anti‐D used by a one‐stage bromelain technique and anti‐D with modified IgG appeared to be relatively insensitive in detecting D u in this study. Polyclonal anti‐D in an enhancement medium and monoclonal anti‐D scored better, although differences exist between the products of various manufacturers. It is suggested that if IAT (D u ) testing is omitted, only anti‐D sera with a high index of detectability of cells expressing weak D antigens should be accepted.