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HLA Class I (Bg) Antigens on Red Cells of SLE Patients: A Serological Study with Polyclonal and Monoclonal Antibodies
Author(s) -
Giles Carolyn M.,
Darke Christopher,
Rowe Graham P.,
Botto Marina
Publication year - 1989
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1989.tb02038.x
Subject(s) - polyclonal antibodies , monoclonal antibody , epitope , human leukocyte antigen , monoclonal , immunology , antibody , antigen , serology , microbiology and biotechnology , chemistry , biology
. The enhanced HLA class I (Bg) on red blood cells (RBC) of many patients with systemic lupus erythematosus has allowed a significant correlation to be made between their HLA‐B types and haemagglutination reactivity with lymphocytotoxic anti‐HLA‐B sera stimulated by pregnancy alone. Therefore the class I expression on these RBC relates to classical, rather than non‐classical, class I gene products. Studies of class I expression on RBC by means of monoclonal antibodies (MAb) to epitopes on the heavy polypeptide chain and β 2 ‐microglobulin (β 2 m) have suggested that the complete extracellular structure is present. The specific effect of chloroquine in ‘stripping HLA’ from RBC had been assumed to support the concept that HLA class I was adsorbed from plasma. However, from our data, we conclude that HLA class I is an intrinsic membrane component. We suggest that the action of chloroquine is to remove β 2 m alone, which prevents normal class I expression and also results in conformational changes to the class I heavy chain, but that it is not capable of removing the membrane‐bound heavy chain.