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Effect of Gamma Irradiation on the Human Immunodeficiency Virus and Human Coagulation Proteins
Author(s) -
Kitchen A.D.,
Mann G.F.,
Harrison J.F.,
Zuckerman A.J.
Publication year - 1989
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1989.tb02033.x
Subject(s) - cryoprecipitate , gamma irradiation , fresh frozen plasma , irradiation , human immunodeficiency virus (hiv) , human plasma , chemistry , virology , virus , coagulation , microbiology and biotechnology , biology , medicine , immunology , biochemistry , chromatography , platelet , fibrinogen , physics , nuclear physics
. The effect of gamma irradiation on HIV and plasma coagulation factors F VIII:C, F VIII:vWF and FIX was studied. Donor plasma was harvested from single donations, frozen and irradiated in the frozen state at target doses from 0 to 40 kG y (0–4 mRad). HIV was inoculated into human plasma and irradiated in a similar manner. A range of other viruses, not suspended in plasma, were also irradiated to establish viral inactivation. An inactivation rate of 0.164 TCID 50 dose/ml/kG y was demonstrated for HIV compared to rates of 0.00173, 0.00526 and 0.00286 log 10 units/ml/kG y for F VIII:C, F VIII:vWF and FIX respectively. The use of gamma irradiation to inactivate infectious agents present in human plasma may eliminate the need for any post‐production viral inactivation methods and provide a means of assuring the safety of as yet untreated products such as cryoprecipitate and fresh frozen plasma.