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A Novel Second‐Generation Polyolefin Container for Storage of Single‐Donor Apheresis Platelets
Author(s) -
Shimizu T.,
Kouketsu K.,
Kamiya T.,
Futagawa H.,
Hirose S.
Publication year - 1989
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1989.tb02022.x
Subject(s) - apheresis , polyolefin , plateletpheresis , platelet , container (type theory) , blood preservation , medicine , chemistry , surgery , materials science , immunology , andrology , organic chemistry , composite material , layer (electronics)
Abstract. A non‐blow‐molded LE‐2 polyolefin (PO) container was developed to store single‐donor apheresis platelet concentrates (PCs) processed by the Haemonetics Plasma Collecting System (PCS) at 22°C for over 1 day. We molded PO containers with heat‐sealing of light‐weight polymer alloy films with a thickness of 0.25 mm. The film was made of polymer blends consisting of polypropylene, styrene ethylene butylene styrene‐block copolymer, and ehtylene ethylacrylate copolymer. The PO container with enough strength and flexibility in routine practice has 2 and 1.6 times higher oxygen and carbon dioxide gas transfer properties than standard polyvinyl chloride (PVC) plastic containers. PCs (1–1.9times10 11 platelets) processed from 450 ml platelet‐rich plasma were stored in 0.6‐liter PO containers at 22°C with flatbed agitation for up to 6 days. The pH of PCs was well maintained at the mean values of 7.0 in PO containers after 6 days in the well‐oxygenated condition. The energy metabolism of stored platelets was determined. Oxygen consumption rates of platelets stored in PO containers averaged 1.5 nmol/min/10 9 platelets. The rates of glucose consumption and lactate production were 0.4 and 0.8 nmol/min/10 11 platelets, respectively. The rates of adenosine triphosphate (ATP) generation of platelets, 9.7 nmol/min/10 9 platelets, in PO containers did not differ from those in the PVC containers. Aggregation responses to adenosine diphosphate and hypotonic shock response of platelets were better maintained in PO containers. The morphological changes into sphere forms with projections and the appearance of unclassified forms were more frequently observed in PO than in PVC containers. Single‐donor apheresis platelet products collected by the PCS system may be stored in a new 0.6‐liter PO container for over 5 days under acceptable conditions.