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Effect of Centrifugation on the Storage Properties of Platelets
Author(s) -
Solberg C.,
Moen P.,
Little C.
Publication year - 1988
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1988.tb05143.x
Subject(s) - centrifugation , platelet , lactate dehydrogenase , chemistry , chromatography , andrology , biochemistry , enzyme , immunology , biology , medicine
. Some of the recommended centrifugation methods for the preparation of platelet concentrates may cause accelerated deterioration of platelets stored in second‐generation containers. The deterioration is characterized by increasing pH, pO 2 and decreasing pCO 2 , a high discharge of lactate dehydrogenase (LDH) and increasing amounts of small particles which have recently been shown to have platelet factor 3 activity [Solberg, C.; Østerud, B.; Little, C.: Thrombosis Res. 48 : 559–565, 1987]. A short first centrifugation (3,270g, 2 min 15 s) yielded platelets with better storage properties than platelet‐rich plasma prepared with longer centrifugation times (2,200 g, 4 min 30 s and 1,100 g, 6 min). By using multivariate data analysis the effect of different platelet concentrations and metabolic parameters can be used to predict the discharge of LDH or the change in morphology.