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Comparison between a Solid‐Phase Low‐Ionic‐Strength Solution Antiglobulin Test and Conventional Low‐Ionic‐Strength Antiglobulin Test: Assessment for the Screening of Antierythrocyte Antibodies
Author(s) -
Guignier F.,
Domy M.,
Angue M.,
Richaud P.,
Chatelain P.
Publication year - 1988
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1988.tb04684.x
Subject(s) - papain , ionic strength , antibody , chemistry , ionic bonding , chromatography , phase (matter) , immunology , medicine , ion , biochemistry , enzyme , aqueous solution , organic chemistry
. A solid‐phase low‐ionic strength salt antiglobulin test (LISS‐SPAT) has been developed using a micro‐plate coated with dried sera as a solid phase. Before coating, the in vitro C3d fragment generation was activated by adding heat‐aggregated immunoglobulin. The LISS‐SPAT was compared with low‐ionic strength conventional antiglobulin test (LISS‐AGT) and also with a test using polybrene or papain microplates. When detecting the IgG and IgM antierythrocyte antibodies the reaction was developed in the same way in LISS‐SPAT and LISS‐AGT. In routine work, the LISS‐SPAT provides a fast, reliable, handy and inexpensive screening of antibodies. This method appears to be an additional method to the papain and polybrene tests in microplates.