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Efficacy of an Immunoglobulin Preparation from HIV Carriers in Preventing HIV Replication in vitro
Author(s) -
Tochikura Tadafumi S.,
Nakashima Hideki,
Uemura Yahim,
Goto Takashi,
Suyama Tadakazu,
Kobayashi Nobuyuki,
Yamamoto Naoki
Publication year - 1988
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1988.tb03888.x
Subject(s) - antibody , virology , virus , in vitro , biology , cytopathic effect , cell culture , antigen , hiv antigens , immunology , viral disease , biochemistry , genetics
. Immunoglobulin samples (HIV‐Ig) were prepared by cold ethanol fractionation of human plasma containing antibody against human immunodeficiency virus (HIV). The ability to prevent viral spreading was studied using either human T‐cell leukemia virus type I (HTLV‐I)‐carrying MT‐4 cells or in a coculture system using MOLT‐4 cells and virus‐producing MOLT‐4/HIV HTLV‐IIIB cells. Treatment of HIV‐infected MT‐4 cells with HIV‐Ig effectively blocked the appearance of antigens of HIV and the virus‐induced cytopathic effect. HIV‐Ig blocked multinucleated giant cell formation in the MOLT‐4 and MOLT‐4/HIV HTLV‐IIB coculture system.