Premium
A Simple Method to Obtain Low Density Marrow Cells for Human Marrow Transplantation 1
Author(s) -
Witte T.,
Plas A.,
Vet J.,
Koekman E.,
Preyers F.,
Wessels J.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb05070.x
Subject(s) - percoll , pancytopenia , centrifugation , bone marrow , differential centrifugation , transplantation , immunology , andrology , medicine , myeloid , microbiology and biotechnology , chromatography , surgery , biology , chemistry
. Removal of more than 99% of the erythrocytes and 74% of the nucleated cells from marrow grafts was achieved by density floatation separation in Percoll gradients with a density of 1.070 g/ml in eight 250‐ml tubes, containing up to 3 × 10 9 nucleated cells per gradient. More than 90% of the myeloid and erythroid progenitor cells were recovered in the low density fraction. It appeared mandatory to use a centrifuge with the possibility of a gradual acceleration and deceleration. Twenty‐five patients received a marrow graft from a histocompatible sibling after additional lymphocyte depletion by counterflow centrifugation, and 5 patients with T lymphoblastic malignancies received an autograft after in vitro purging with immunotoxins. All evaluable patients engrafted within normal limits, except 1 patient with an autoimmune pancytopenia who responded to steroids and 1 patient with a CMV infection. Four patients died too early for complete evaluation. The described separation method is easy, cheap and requires only 2 h for the complete processing of a marrow graft.