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Radio‐Immunoassay of the Functional Activity of Anti‐D (Rh) Preparations using a Human Monoclonal 125 I‐Labelled Anti‐D
Author(s) -
HughesJones N.C.,
Dawes B.,
Gorick B.D.,
Melamed M.D.,
Pepper R.,
Thompson K.M.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb04945.x
Subject(s) - monoclonal antibody , immunoassay , antigen , agglutination (biology) , antibody , chemistry , rh blood group system , microbiology and biotechnology , immunology , medicine , biology
. The prophylactic effect of anti‐D in the prevention of maternal immunization to the D antigen of the Rh system depends in the first instance on its ability to bind to fetal Rh‐positive red cells. The functional activity of the injected anti‐D in this respect is dependent on both the plasma concentration and functional affinity constant of the antibody, and both factors should be taken into account in assessing IgG anti‐D preparations for clinical use. A radio‐immunoassay utilizing 125 ‐I‐labelled human monoclonal anti‐D has been developed which measures the amount of antibody bound to red cells after the addition of an aliquot of the test anti‐D preparation. The assay is thus a functional test in that it measures the ability of the anti‐D to react with the antigen. The functional activity of the test anti‐D is compared to that of the international standard, and the concentration is then expressed in terms of μg‐equivalents, that is, its equivalence in functional terms to the stated amount of international standard. The radio‐immunoassay was compared to the currently used agglutination assay, and the results were found to give good agreement in 18 out of the 21 samples assayed.