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Phagocytosis of Erythrocytes Sensitized with Known Amounts of IgGl and IgG3 Anti‐Rh Antibodies
Author(s) -
Zupańska B.,
Thompson E.,
Brojer E.,
Merry A.H.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb04926.x
Subject(s) - phagocytosis , antibody , biology , microbiology and biotechnology , monocyte , chemistry , immunology
. Phagocytosis was investigated using human peripheral monocytes and erythrocytes sensitized with known amounts of subclass‐specific IgG anti‐Rh antibodies. The erythrocyte‐bound IgG was quantitated by a radiometric antiglobulin test. This evaluation revealed the following: (1) there is a relationship between phagocytosis and the number of erythrocyte‐bound IgG molecules; (2) phagocytosis is IgG subclass‐dependent, since a similar degree of phagocytosis is observed with fewer IgG3 than IgGl molecules and also the minimum number of IgG3 molecules for phagocytosis is 150–640, whilst for IgGl the minimum is 1,230‐4,020; (3) the minimum levels of sensitization for phagocytosis should be detectable by the serological antiglobuiin test; (4) the phagocytosis assay is no more sensitive than the monocyte rosette assay for the detection of anti‐Rh alloantibodies, and (5) phagocytosis of adherent erythrocytes observed by video‐enhanced microscopy indicated that erythrocytes may adhere to monocytes for a considerable time before phagocytosis, but that phagocytosis itself was rapid.