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Amount and Type of Leukocytes in ‘Leukocyte‐Free’ Red Cell and Platelet Concentrates
Author(s) -
Vakkila Jukka,
Myllylä Gunnar
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb04922.x
Subject(s) - platelet , buffy coat , immunology , monoclonal antibody , red cell , filtration (mathematics) , microbiology and biotechnology , polymorphonuclear leukocyte , chemistry , medicine , antibody , biology , biochemistry , in vitro , mathematics , statistics
. The exact number of leukocytes remaining in ‘leukocyte‐free’ red cell and platelet concentrates cannot be measured by standard methods. We have therefore developed methods to harvest all the leukocytes from blood components. The leukocytes were then counted and identified using monoclonal antibodies. The leukocyte‐free red cell concentrates were prepared by combining buffy coat removal and filtration through a Cellselect filter. The mean number of leukocytes per unit was 1.0 times 10 5 . Most of the leukocytes were granulocytes and T cells. Only a few B cells or monocytes could be detected. Leukocyte‐free platelets were prepared by filtering 4 units of PC through a cotton‐wool (Imugard) filter. The mean number of leukocytes per PC unit was 0.4 times 10 5 of which 85–95% were T cells.