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Estimation of the Number of Binding Sites for a Murine Monoclonal Anti‐Lu b on Human Erythrocytes
Author(s) -
Merry A. H.,
Gardner B.,
Parsons S. F.,
Anstee D. J.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb04915.x
Subject(s) - microbiology and biotechnology , phenotype , monoclonal antibody , biology , antibody , antigen , gene , genetics
. The number of Lu b antigen sites on the human erythrocyte membrane, as recognised by the murine monoclonal antibody BRIC‐108, has been determined. The number of antibody molecules bound per cell on one example of cells of the phenotype Lu(a‐b‐) with recessive inheritance was an average of around 200 in replicate determinations, which probably represents non‐specific antibody binding. A similar number of antibody molecules to this bound to trypsin‐ or pronase‐treated normal cells or cells of the Lu(a‐b‐) phenotype associated with the inheritance of the X‐borne gene, XS2. The number of binding sites on three examples of cells of the phenotype Lu(a‐b‐) with dominant inheritance was from 440 to 690. A variation in the number of binding sites per cell from 1,640 to 4,070 was found in five individuals with the phenotype Lu(a‐b+) and from 850 to 1,820 in four individuals with the phenotype Lu(a+b+). Four individuals with the Lu(a+b‐) phenotype had an average of 480 binding sites per cell. The Lu b antigen therefore appears to a have low‐site density and a variable level of expression on the erythrocyte surface.