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Identification of the Yuk b Allo‐Antigen on Platelet Glycoprotein IIIa
Author(s) -
Santoso S.,
Shibata Y.,
Kiefel V.,
MuellerEckhardt C.
Publication year - 1987
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1987.tb04913.x
Subject(s) - platelet , antigen , glycoprotein , microbiology and biotechnology , antibody , immune system , kilodalton , chemistry , biology , immunology
. The glycoprotein (GP) localization of a new platelet‐specific allo‐antigen Yuk b is described. The antibody was isolated from serum of a patient with neonatal allo‐immune thrombocytopenia. In immunoblot procedure, it bound exclusively to platelet GP IIIa, like anti‐Pl A1 , while the known anti‐Bak a and anti‐Lek a reacted with GP IIb. Analysis of GP from chymotrypsin‐treated platelets with anti‐Yuk b revealed no binding in the 68‐kilodalton position while anti‐Pl A1 did. Thus, unlike the P1 A1 antigen, the Yuk b determinant either resides on the 30‐kilodalton fragment of GP IIIa or it is destroyed by chymotrypsin treatment.